Dynamic Dialysis
How it works
Dynamic dialysis is an exciting new technology that utilizes fluid dynamics to increase purification efficiency and improve large buffer handling, ideal for the production of fragile proteins, viscous fluids and polymer gels, such as hyaluronic acid. These closed, high-efficiency systems offer high concentration gradient dialysis without manual buffer changes and in-process sample handling.
Static dialysis
Requires labor & planning
Manual buffer changes
Low separation efficiency
Limited scalability
Multiple sample handling
Open system
Dynamic dialysis
Walk away convenience
Continuous buffer changes
Maximal separation efficiency
Easy scalability
No in-process membrane handling
Closed system
Flow comparison
Static dialysis
Inefficient dialysis
Manual buffer changes lead to inefficient dialysis
Passage slows as solutes approach equilibrium
Requires buffer change to reestablish concentration gradient to drive dialysis
Longer dialysis duration may be required
Dynamic dialysis
More efficient dialysis
Continuous buffer changes flow leads to more efficient dialysis
Solutes never approach equilibrium
Maintains maximum concentration gradient and solute passage
Shorter overall dialysis duration possible
How dynamic dialysis works
Continuous buffer flow without the need for sample handling or manual buffer changes. Contaminants are expelled to waste. Clearance of contaminants from the tank helps maintain high concentration gradient.
Model of dynamic
dialysis process
Efficient scale-up of large colume dynamic-dialysis for purification. GMP batch production of shear-sensitive protein.
Flow comparison
Recirculation mode
More efficient passage of dilute solute samples
Fast flow to promote buffer mixing (0.5-1.0 L/min)
Recirculation maintains buffer volume
Recirculation mode
Continuous buffer changes flow leads to more efficient dialysis
Solutes never approach equilibrium
Maintains maximum concentration gradient and solute passage
Shorter overall dialysis duration possible
