Dynamic Dialysis

How it works

Dynamic dialysis is an exciting new technology that utilizes fluid dynamics to increase purification efficiency and improve large buffer handling, ideal for the production of fragile proteins, viscous fluids and polymer gels, such as hyaluronic acid. These closed, high-efficiency systems offer high concentration gradient dialysis without manual buffer changes and in-process sample handling.

Static dialysis

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Requires labor & planning
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Manual buffer changes
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Low separation efficiency
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Limited scalability
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Multiple sample handling
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Open system

Dynamic dialysis

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Walk away convenience
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Continuous buffer changes
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Maximal separation efficiency
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Easy scalability
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No in-process membrane handling
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Closed system

Flow comparison

Static dialysis

Inefficient dialysis

Manual buffer changes lead to inefficient dialysis
Passage slows as solutes approach equilibrium
Requires buffer change to reestablish concentration gradient to drive dialysis
Longer dialysis duration may be required
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Dynamic dialysis

More efficient dialysis

Continuous buffer changes flow leads to more efficient dialysis
Solutes never approach equilibrium
Maintains maximum concentration gradient and solute passage
Shorter overall dialysis duration possible

How dynamic dialysis works

Continuous buffer flow without the need for sample handling or manual buffer changes. Contaminants are expelled to waste. Clearance of contaminants from the tank helps maintain high concentration gradient.

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Model of dynamic
dialysis process

Efficient scale-up of large colume dynamic-dialysis for purification. GMP batch production of shear-sensitive protein.

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Flow comparison

Recirculation mode

More efficient passage of dilute solute samples
Fast flow to promote buffer mixing (0.5-1.0 L/min)
Recirculation maintains buffer volume
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Recirculation mode

Continuous buffer changes flow leads to more efficient dialysis
Solutes never approach equilibrium
Maintains maximum concentration gradient and solute passage
Shorter overall dialysis duration possible
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Do you have any questions or do you want to get a quote?
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Elliot Schupak
European Director
Mobile: +33 (0) 768 490 441
Telephone: +41 (0) 27 329 09 36
e.schupak@defimedica.com
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